1、the idea of "each parent contributes particles or genetic unites to the offspring" was first proposed by: a、morgan b、mendel c、avery d、watson
2、molecular biology is a study of gene structure and function at the molecular level.
核酸随堂测验
1、what are the properties of genetic materials? a、it must be able to hold information for how to make proteins b、it must be stable c、it must have some capacity for change. d、it must be able to be copied.
2、when nucleotides are polymerized, the triphosphate group of one nucleotide reacts with the 3’-oh (hydroxyl) group of another nucleotide.
基因功能概论随堂测验
1、proteins are the final products of most genes
2、the tertiary structures of peptides usually have two forms,α helix and β sheet.
第二章 原核生物转录：机理与调控
原核生物转录机理随堂测验
1、during transcription, the newly produced mrna molecule is a perfect complement of the template strand.
2、the core enzyme of rna polymerase can recognize the promoter accurately so that transcription can begin in the right place.
协同调控与乳糖操纵子随堂测验
1、transcription of the lac operon is weak when camp levels are low.
色氨酸操纵子随堂测验
1、when tryptophan levels in the cell are low, . a、the ribosome slows down while translating the leader region. b、the terminating hairpin loop forms c、the ribosome never attaches to the leader region d、the ribosome continues transcription of the trp operon without stopping
2、the trp operon also appear in eukaryotes.
第三章 真核生物转录：机理与调控
真核rna聚合酶与启动子随堂测验
1、which of the following elements is no included in eukaryotic promoter? a、the tata box b、the initator c、the -10 box d、dpe
2、eukaryotes use three different rna polymerases, each of them transcribes different classes of genes
通用转录因子与转录起始随堂测验
1、the first tfii to bind the promoter is tfiid
2、different rnap share the same general transcription factors.
特异转录因子与转录调控随堂测验
1、activators and repressors are proteins, enhancers and silencers are dna sequences.
2、an repressor can recruit enzyme that loosens dna from nucleosome
特异转录因子结构随堂测验
1、which of the follwing motifdcontains a beta-sheet? a、zinc finger b、helix-turn-helix c、helix-loop-helix d、leucine zipper
2、most dna-binding proteins recognize specific dna sequences at the major groove
第四章 翻译
遗传密码与原核生物翻译随堂测验
1、there are three start codons in genetic code.
2、in prokaryotes, translation can start at any of the aug sequence.
真核生物翻译随堂测验
1、in eukaryotes, the initiator trna carries nomal methionine.
2、one trna can recognize only one codon.
第五章 真核生物mrna的修饰
真核生物mrna的修饰：加帽与加尾随堂测验
1、the 5' cap helps to prevent degradation of the mrna at the 5' end.
2、the poly a tail can be added at any place of the 3' end
真核生物mrna的剪接与编辑随堂测验
1、most genes only have one intron.
2、the first two bases on the 5' side of an intron are almost always ( ).
第六章 真核生物基因表达调控
组蛋白与转录随堂测验
1、histones carry positive charges, and dna is negative charged.
2、methyl groups can be added to the tails of histone.
转录后调控随堂测验
1、polyadenylation can be regulated, so alternative polyadenylation is a kind of post-transcriptional regulation.
2、the ( ) can selectively choose which mrna exit the nucleus, therefore which get to be expressed.
rna 稳定性及翻译调控随堂测验
1、what is true of mirna and sirna? a、they usually target the same mrna b、they both work in conjuction with risc complex. c、they both are processed by drosha. d、they are present in prokaryrotes.
2、translational regulation produces quick changes in protein production.
mrna 的定位随堂测验
1、the localization of proteins without signal peptides, depends to a great extent on where their mrna is translated.
2、to concerntrate beta-actin protein on the moving edge of a fibroblast, the beta-actin mrna can not be ( ) before arrival at the edge.
第七章 突变与突变修复
突变类型随堂测验
1、alkylating agents add chemical groups to dna bases.
2、uv light can cause two purines to become covalently linked
突变后果随堂测验
1、an insertion of a nucleotide in to a gene is more serious than the deletion of a nucleotide.
2、if the mutation occurs such that one codon is changed to another codon that codes for a different amino acid, it is called a ( )
突变修复随堂测验
1、the two main errors that occur during dna replication, mismatched bases and strand slippage, are both corrected by a mechanism called mismatch repair (mmr).
2、ner and mmr recognize the same kinds of errors
第九章 重组
同源重组-交换机理随堂测验
1、holiday junctions msy be resolved in more than one way.
2、non-crossover resolution of holiday junction would be used in double stranded break repair.
同源重组-断裂与修复机制随堂测验
1、crossing over occurs during meiosis in e. coli
非同源重组随堂测验
1、反转录转座子需要转录酶来复制他们自己。
2、proteins are ususally required for transposons to insert into a chromosome.
第八章 dna复制
半保留复制与半不连续复制随堂测验
1、真核和原核生物dna复制都是以半保留复制与半不连续复制的方式进行。
2、okazaki fragments are never joined together
dna 复制的酶和蛋白质随堂测验
1、大肠杆菌dna复制的主要酶是dna聚合酶ⅲ，dna聚合酶ⅰ主要负责修复。
端粒与端粒酶随堂测验
1、端粒的长短和细胞分裂的次数有关
2、端粒酶中含有一小段dna能够作为模板指导端粒的合成
第十章 分子生物学主要技术和应用
聚合酶链式反应随堂测验
1、primers are required in pcr.
2、本次新冠肺炎的核酸筛查就是基于pcr的检测。
分子杂交及印迹随堂测验
1、northern blot is mainly used to detect a targeted mrna expression in biological samples
2、a （ ） is a polynucleotide fragment linked with a special detectable marker
基因工程随堂测验
1、限制性内切酶可以在任意位置切开dna双链。
2、（ ）are necessary to insert target genes into prokaryotic or eukaryotic cells and express the target proteins efficiently